Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2016 Jul 1;6:29045. doi: 10.1038/srep29045

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2016, Macmillan Publishers Limited

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

PMC Copyright notice

(A) AVP1 expression impairs vacuole fragmentation. Wildtype cells (BY4741) and cells expressing GFP-AVP1 (SVY12) were grown logarithmically in SC medium and labeled with FM4-64. NaCl was added to a final concentration of 0.5 M NaCl (t = 0 min) to induce vacuole fragmentation. During the following 12 min of incubation at 30 °C, the cells were analyzed by taking stacks of confocal images. Maximum-intensity projections of FM4-64 fluorescence in the stacks are shown, covering 6 μm in the z-direction. (B) Classification of the cells shown in panel A according to the number of vacuoles per cell. 200–400 cells were analyzed per strain (n = 3–5).