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. 2016 Jul 1;6:29073. doi: 10.1038/srep29073

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Copyright © 2016, Macmillan Publishers Limited

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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Transverse uterine sections from juvenile (3 weeks old) 129S1/SvImJ Kiss1^−/− (A,C) and WT littermates (B,D) were analyzed for FOXA2-positive endometrial glands and uterine size. Sections shown in (A,B) were analyzed using the anti-FOXA2 antibody at a concentration of 1.8 μg IgG/ml. while those in (C,D) were analyzed using anti-FOXA2 antibody at a concentration of 0.18 μg IgG/ml. Glands were quantified and uterine growth was determined by measuring the average area (μ²) of a transverse uterine section (excluding the uterine lumen) per uterine horn; data are displayed graphically (E,F). M: mesometrial; AM: anti-mesometrial. The number of independent investigations is reported in the figure and the data are shown as mean ± SEM.