Fig. 4.
Effects of MHT on PDT, drug, or PCI on spheroid growth 24 hours after initiation, spheroids undergoing PDT or PCI were incubated with1 μg/ml AlPcS2a for 18 h. Drug concentrations used were 0.6 and 0.12 μg/ml for BLM (Fig. 4 a, c) and 5-FU (Fig. 4 (b, c) respectively. Four hours following drug addition PDT/PCI light treatment at fluence levels of 0.3 J/cm2 or 1.5 J/cm2 (F98 Fig. 4 a, b) or (ACBT Fig. 4 c, d). Light treatment was done at 37, 40 or 44°C following a 45 min MHT incubation. Incubation was continued at 37°C for an additional 14 days. Each data point represents spheroid volume after 2 weeks in post treatment culture as a % of 37°C controls at 14 days and is the mean of 3 experiments. Error bars denote standard errors. Asterisks (*) denote significant differences (p<0.05) compared to control values.