α1 (A), τ1 (B), and τ2 (C) error for simulated two-component fluorescence decay curves analyzed in SPCImage. α1 (D), τ1 (E), and τ2 (F) error for simulated two-component fluorescence decay curves analyzed in SLIM Curve. Average of 256 simulations, 10,000 photons/curve (SNR = 100).