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. 2016 Jun 17;48(6):e240. doi: 10.1038/emm.2016.39

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Copyright © 2016 KSBMB.

This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivs 4.0 International License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-nd/4.0/

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PLC-β3 regulates proliferation, migration and tube formation of HUVECs. (a) Heart, lung, kidney, liver, spleen and retina were isolated from either WT or PLC-β3 knockout mice, and expression of PLC-β1, PLC-β2, PLC-β3 and PLC-β4 was verified by western blot analysis with the indicated antibodies. (b) HUVECs and VSMC were harvested and expression of PLC-β1, PLC-β2, PLC-β3 and PLC-β4 was verified by western blot analysis with the indicated antibodies. (c, d) PLC-β3 was silenced in HUVECs and EGM-2-dependent proliferation and migration was measured. (e) PLC-β3 was silenced in HUVECs and EGM-2-induced tube formation was visualized on bright-field microscope at × 10 magnification. Data are means±s.e.m. of three independent experiments (n=3 for each experiment). Asterisks indicate statistical significance (P<0.05). Scale bar, 500 μm.