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. 2016 Jun 3;48(6):e237. doi: 10.1038/emm.2016.43

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Copyright © 2016 KSBMB.

This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivs 4.0 International License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-nd/4.0/

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Generation of mALK2-iPSC from mALK2-hDF. (a) Alkaline phosphatase (AP) staining of primary hFFn-iPSC and mALK2-iPSC. (b) Number of primary iPSC colonies generated from 1.0 × 10⁵ normal human foreskin fibroblasts (hFFn) (black bar) or mALK2-hDF (gray bar). (c) AP staining of hFFn- and mALK2-iPSCs cultured on a layer of mouse embryonic fibroblast-feeder cells. (d) Immunofluorescence detection of pluripotency markers NANOG, OCT4 and TRA-1-81 in the hFFn-iPSC or mALK2-iPSC. (e) Relative mRNA expression of pluripotency genes OCT4, SOX2, DNMT3B and ESG1 in normal hFFn, mALK2-hDF, hFFn-iPSC and mALK2-iPSC clones #1 and 2. Gene expression was normalized to GAPDH expression. Fold change of pluripotency genes in hFFn, mALK2-hDF, mALK2-iPSC1 and mALK2-iPSC2 relative to hFFn-iPSC (mean±s.d.). (a) Scale bars=50 μm; 200 μm (c, d). BF, brightfield image.