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. 2016 Jun 3;48(6):e237. doi: 10.1038/emm.2016.43

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Copyright © 2016 KSBMB.

This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivs 4.0 International License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-nd/4.0/

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Impaired clonogenic potential of single-cell-dissociated mALK2-iPSC. (a) Green fluorescent protein (GFP)-expressing hFFn-iPSCs or mALK2-iPSCs on Matrigel or feeder layer 1 day post transfection. (b) Magnified image of c. (c) GFP expression and alkaline phosphatase (AP) staining in hFFn-iPSCs and mALK2-iPSCs 15 days post transfection. (d) Counting cell clumps of c. Scale bars=25 μm (a); 50 μm (b). hFFN, human foreskin fibroblasts; iPSC, induced pluripotent stem cell.