Figure 2.

Mice on a long-term high fat diet develop a diabetic phenotype. Young (1.5-month-old) male mice were placed on a high fat (HF) diet or control low fat (LF) diet. (a) Prior to initiation of dietary intervention there were no differences in body weight between groups. (b) HF diet caused an increase in body weight after 6 weeks on the diet which persisted as weight gain plateaued after 12 weeks (*p < 0.001 vs. LF). N = 30 per group. (c) After 3 months on the HF diet, fasting blood glucose levels were slightly elevated (time 0), however; clearance of glucose was greatly impaired (p < 0.001 vs. LF), N = 30 per group. (d) After 6 months on the HF diet, fasting blood glucose levels were significantly elevated and glucose clearance was significantly impaired in HF compared to LF diet mice, regardless of VCI or sham surgery (p < 0.01 vs. LF sham). N = 13–15 per group.