Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
Purification of BCAI expressed in recombinant E. coli
| Purification step | Protein(mg) | Total activity(U) | Specific activity(U mg-1) | Yield(%) | Purification fold |
|---|---|---|---|---|---|
| Crude extract | 39.4 | 89.9 | 2.3 | 100 | 1 |
| Heat treatment | 15.7 | 78.4 | 5.0 | 87.2 | 2.2 |
| HisTrap HP 5 mL column | 2.3 | 18.3 | 8.0 | 20.3 | 3.5 |
Then enzyme activity was measured using L-arabinose as a substrate
