Table 1.
A list of Escherichia coli strains used in this study. E. coli and ETEC strains were used for PCR amplification of adhesin tip and adhesive subunit genes and also for antibody adherence inhibition assays. Recombinant E. coli strains expressing each adhesin tip or adhesive subunit constructed in this study were also included.
| Strain | Relevant characteristics | Source |
|---|---|---|
| H10407 | O78:H11; CFA/I, LT, STa | Johns Hopkins University |
| THK38/pEU405 | CS1 | Emory University (47) |
| DH5α/pEU588 | CS2 | Emory University (48) |
| E116 (E19446) | CS3, LT, STa | University of Gothenburg |
| E106 (E11881/9) | CS4/CS6, LT, STa | University of Gothenburg |
| UM 75688 | CS5/CS6, LT, STa | Johns Hopkins University |
| JF2423 ETP98066 | CS6, LT, STa | Washington University |
| JF2101 | CS21, EtpA, STa | Washington University |
| JF2318 ETP050008 | EtpA, STa | Washington University |
| CFA/I knockout | ΔCFA/I H10407, EtpA, LT, STa | University of Maryland (46) |
| 9473 | CfaE (CFA/I) in pET28α/ BL21 Kan+ | this study |
| 9474 | CooD (CS1) in pET28α/ BL21 Kan+ | this study |
| 9475 | CotD (CS2) in pET28α/BL21 Kan+ | this study |
| 9505 | CstH (CS3) in pET28α/BL21 Kan+ | this study |
| 9504 | CsaE (CS4) in pET28α/BL21 Kan+ | this study |
| 9533 | CsfD (CS5) in pET28α/BL21 Kan+ | this study |
| 9506 | CssB (CS6) in pET28α/BL21 Kan+ | this study |
| 9468 | LngA (CS21) in pET28α/BL21 Kan+ | this study |
| 9507 | EtpA in pET28α/BL21 Kan+ | this study |
| 9450 | adhesin tip MEFA in pET28α/BL21 Kan+ | this study |