Figure 3:
EGCG treatment reduces stem cells number. Effect of EGCG on the total cell number was analyzed by growing C3H10T1/2 cells in the presence of 1 μM or 5 μM EGCG. About 20,000 cells were plated in six well plate. Twenty four hours following treatment the cells from control and EGCG treated cells were trypsinized and counted. At both the concentrations of EGCG the cell numbers were found to be significantly reduced as compared to control cells. The values represent mean ± SEM for three independent experiments.