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. 2016 Jun 15;6(6):160042. doi: 10.1098/rsob.160042

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© 2016 The Authors.

Published by the Royal Society under the terms of the Creative Commons Attribution License http://creativecommons.org/licenses/by/4.0/, which permits unrestricted use, provided the original author and source are credited.

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Figure 6. — α-terpineol fails to activate five N-terminal deletion mutants of TmTRPA1b. (a) The amino acid sequences deleted in five TmTRPA1b mutants (Δ105–134, Δ77–134, Δ49–134, Δ21–134 and Δ84–92) are indicated by dashed lines. The first amino acid of AR1 (R) is marked with purple. (b) The traces show the responses of five N-terminal deletion mutants of TmTRPA1b to 1 mM α-terpineol followed by 0.5 mM carvacrol tested by calcium imaging. Red and blue bars show the periods when α-terpineol and carvacrol were added and then washed off, respectively. Arrows show the time points when ionomycin was added.