1. Characteristics of Studies.

Study	Number of participants	Method of granulocyte procurement	Dose of granulocyte transfusions Median (Range)	Frequency of granulocyte transfusions	Granulocytes Irradiated	Period of observation post onset of fever	Donor pre‐medication	Donor selection
Alavi 1977	31	Filtration leukapheresis	3.3 x 1010/m2 children (Not reported) 3.2 x 1010/m2 adults (Not reported)	Daily	Not reported	1 to 24 hours	Hydrocortisone	Red cell compatibility alone
Bow 1984	24	Discontinous flow centrifugation	Mean 0.49 x 1010/m2 SD 0.20a	Not reported	Not reported	72 hours	Dexamethasone	HLA compatibility WBC compatibility/crossmatch
Herzig 1977	27	Continous flow centrifugation	0.4 x 1010/m2 (0.2 to 0.8)	Daily	Yes 1500 to 2500 rads	variableb	None	WBC compatibility/crossmatch
		Filtration leukapheresis	1.7 x 1010/m2 (0.6 to 4.6)
Higby 1975	36	Filtration leukapheresis	2.2 x 1010/m2 (1.1 to 5.0)	Daily	Not reported	48 hours	Dexamethasone	WBC compatibility/crossmatch
Klastersky 1983	39	Not reported	> 1.0 x 1010/m2 (Not reported)	Daily	Not reported	24 hours	Not reported	Not reported
Price 2015	97	Continuous flow centrifugation	5.5 x 1010 (IQR 2.6 to 7.3)	Daily	Yes 1500 to 2500 cGy	variablec	G‐CSF and Dexamethasone	Red cell compatibility alone
Scali 1978	25	Continous flow centrifugation	2.9 x 1010	Daily	Not reported	24 hours	Not reported	Red cell compatibility alone
Seidel 2008a	74	Continous flow centrifugation	4.6 x 1010 (0.84 to 11.2)d	Alternate days	Yes 30Gy	Not reported	G‐CSF	Not reported
Vogler 1977	30	Continous flow centrifugation	2.7 x 1010 (Not reported)	At least 4 transfusions in an 8 day period	Not reported	variablee	None, hydrocortisone, or dexamethasone	HLA compatibility WBC compatibility/crossmatch
Winston 1982a	95	Discontinous flow centrifugation	0.5 x 1010 (0.1 to 2.7)	Daily	No	variablef	None	Red cell compatibility alone

^aConverted to per m² by dividing by the estimated adult body surface area (1.79) in adult patients according to Sacco 2010.

^bWithin 24 hours of a positive blood culture result.

^cEligibility criteria changed during the trial. Initially treatment was within 24 hours of a proven or probable bacterial fungal infection. After 31 months this was liberalised within one week. Time from eligibility to first transfusion was 2.3 ± 1.2 days.

^dAssuming weight is 70kg.

^eWhen patient had received at least 72 hours of appropriate antibiotics (bactericidal in vitro) to treat organism identified on culture. Five participants had an infection for more than 10 days (2 control, 3 granulocyte transfusions); 13 participants had an infection for 5 to 10 days (5 control, 8 granulocyte transfusions); and 12 participants had an infection for 3 to 5 days (6 control, 6 granulocyte transfusions).

^fWithin 24 hours of a positive blood culture result, the appearance of an infiltrate on chest X‐ray, or the initial development of a cellulitis or abscess.