Table 1.
Virus | Forward primer* | Reverse primer* | Probe** |
---|---|---|---|
HIV-1 | GCACTTTAAATTTTCCCATTAGTCCTA | CAAATTTCTACTAATGCTTTTATTTTTTC | f-AAGCCAGGAATGGATGGCC-q |
HSV-1 | GGGCAGCGTTGATAGGAATTTA | TGTTTTCGGCGCTACCGA | f-CTCCCGGTACAGGTCGGCGTTG-q |
HSV-2 | CGCCAAATACGCCTTAGCA | AGGTTCTTCCCGCGAAAT | f-CTCGCTTAAGATGGCCGATCCCAATC-q |
CMV | AGGTCTTCAAGGAACTCAGCAAGA | CGGCAATCGGTTTGTTGTAAA | f-AACCCGTCAGCCATTCTCTCGGC-q |
EBV | CGGAAGCCCTCTGGACTTC | CCCTGTTTATCCGATGGAATG | f-TGTACACGCACGAGAAATGCGCC-q |
HHV-6 | GTTAGGATATACCGATGTGCGTGAT | TACAGATACGGAGGCAATAGATTTG | f-TCCGAAACAACTGTCTGACTGGCAAAA-q |
HHV-7 | AGCGGTACCTGTAAAATCATCCA | AACAGAAACGCCACCTCGAT | f-ACCAGTGAGAACATCGCTCTAACTGGATCA-q |
HHV-8 | TCGGTGGCGATGCTTTAGAC | TGAAGCAGACGATGCTTTGC | f -TCGTAACCCCCGTCTACTTTCCCCG-q |
All sequences are given from 5’ to 3’ end.
All our probes have 5’ 6-FAM™ reporting dye (f-) and 3’ Black Hole Quencher® (-q).