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. Author manuscript; available in PMC: 2016 Jul 1.
Published in final edited form as: Bio Protoc. 2015 May 5;5(9):e1465. doi: 10.21769/bioprotoc.1465

Table 1.

Primers and Probes for HIV and different Herpesviruses, adapted from Gianella et al. (2013b)

Virus Forward primer* Reverse primer* Probe**
HIV-1 GCACTTTAAATTTTCCCATTAGTCCTA CAAATTTCTACTAATGCTTTTATTTTTTC f-AAGCCAGGAATGGATGGCC-q
HSV-1 GGGCAGCGTTGATAGGAATTTA TGTTTTCGGCGCTACCGA f-CTCCCGGTACAGGTCGGCGTTG-q
HSV-2 CGCCAAATACGCCTTAGCA AGGTTCTTCCCGCGAAAT f-CTCGCTTAAGATGGCCGATCCCAATC-q
CMV AGGTCTTCAAGGAACTCAGCAAGA CGGCAATCGGTTTGTTGTAAA f-AACCCGTCAGCCATTCTCTCGGC-q
EBV CGGAAGCCCTCTGGACTTC CCCTGTTTATCCGATGGAATG f-TGTACACGCACGAGAAATGCGCC-q
HHV-6 GTTAGGATATACCGATGTGCGTGAT TACAGATACGGAGGCAATAGATTTG f-TCCGAAACAACTGTCTGACTGGCAAAA-q
HHV-7 AGCGGTACCTGTAAAATCATCCA AACAGAAACGCCACCTCGAT f-ACCAGTGAGAACATCGCTCTAACTGGATCA-q
HHV-8 TCGGTGGCGATGCTTTAGAC TGAAGCAGACGATGCTTTGC f -TCGTAACCCCCGTCTACTTTCCCCG-q
*

All sequences are given from 5’ to 3’ end.

**

All our probes have 5’ 6-FAM™ reporting dye (f-) and 3’ Black Hole Quencher® (-q).