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. Author manuscript; available in PMC: 2016 Jul 1.
Published in final edited form as: Bio Protoc. 2015 May 5;5(9):e1465. doi: 10.21769/bioprotoc.1465
Dilution Dilution factor Copies per well (per 10 μl)
D5 10* 8 × 104
D6 10* 8 × 103
D7 10* 8 × 102
D8 10* 8 × 101
D9 10* 8
D10 10* 0.8
D11 10* 0.08
Neg ctrl H2O 0
*
5 μl in 45 μl 10 mM Tris buffer