Skip to main content
. 2016 Jul 1;11(7):e0158408. doi: 10.1371/journal.pone.0158408

Fig 2. Characteristics of human lymphatic endothelial cells.

Fig 2

(A) Phase contrast microscopy image showing the morphology of HDLECs used in this study. Scale bar: 1 μm. (B) Real-time PCR to confirm the expression of VEGFR-3 mRNA in human HDLECs and HUVECs. Histograms represent the relative expression of VEGFR-3 mRNA. Results are expressed as means ± SEM of three independent experiments; ***p < 0.001, t-test. PCR (C) and immunoblot analysis (D) to confirm the expression of leptin receptor (Ob-R) mRNA (C) and protein (D) in HDLECs and HUVECs. (B and C) GAPDH was used as an internal control. bp: base pair. (D) β-actin was used as a loading control.