Table 2. List of primers for polymerase chain reaction used in this study.

Gene	Forward primer (5’ → 3’)	Reverse primer (5’ → 3’)	Product size (bp)
Ob-R	GCCGAAAGCCAGAGACAACC	GCTGGGAATGGGCACGATAC	392
IL-1β	GGGCCTCAAGGAAAAGAATC	TTCTGCTTGAGAGGTGCTGA	205
IL-6	TACCCCCAGGAGAAGATTCC	TTTTCTGCCAGTGCCTCTTT	175
IL-8	GTGCAGTTTTGCCAAGGAGT	CTCTGCACCCAGTTTTCCTT	196
TNF-α	CCCCAGGGACCTCTCTCTAA	TGAGGTACAGGCCCTCTGAT	212
IL-6R	CATTGCCATTGTTCTGAGGTTC	AGTAGTCTGTATTGCTGATGTC	260
GAPDH	GAGTCAACGGATTTGGTCGT	TTGATTTTGGAGGGATCTCG	238

Ob-R, leptin receptor; IL, interleukin; TNF, tumor necrosis factor; IL-6R, IL-6 receptor; GAPDH, glyceraldehyde-3-phosphate dehydrogenase; bp, base pair.