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. 2016 Jul 1;11(7):e0158579. doi: 10.1371/journal.pone.0158579

Fig 5. Bioluminescence emission of selected aequorin variants in an in vivo mouse model.

Fig 5

Mice received a 5 μL intrastromal or antechamber injection in the right eye with variant aequorin and the left eye with HEPES Buffer. (A,B) Aequorin injected intrastromally at a concentration of 2.7 x 10−5 M, 30 s exposure. (C) Aequorin with L-4-methoxyphenylalanine at positions 82 and 86. (D) HEPES Buffer only in both eyes. (E) Aequorin injected in the antechamber at a concentration of 3.2 x 10−5 M, 60 s exposure. (F) Aequorin with L-4-iodophenylalanine at positions 82 and 86. Images generated with Matlab R2014b and LivingImage 4.4. Reprinted from [47] under a CC BY license, with permission from Open Access Dissertations, original copyright 2015.