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. Author manuscript; available in PMC: 2016 Sep 1.
Published in final edited form as: Structure. 2015 Jul 30;23(9):1665–1677. doi: 10.1016/j.str.2015.06.022

Table 1.

ITC binding experiments using various LPHN3 and FLRT3 constructs. For each pair, at least three experiments were recorded.

Deletion constructs of LPHN3 and FLRT3
KD (nM) LPHN3 GAIN-HR OLF-RBL OLF
FLRT3 31±7.9 No binding 43.3±13.8 n.d.
FN3 No binding n.d. n.d. n.d.
LRR n.d. n.d. 34.2±7.5 16.9±15.9
Amino-acid substitutions in LPHN3-OLF and FLRT3-LRR
KD (nM) LPHN3-OLF LPHN3-OLF D332A LPHN3-OLF Y323A LPHN3-OLF A313S
FLRT3-LRR 17 170 No binding 19
FLRT3-LRR R117T 27 n.d. n.d. n.d.
FLRT3-LRR F160N No binding n.d. n.d. n.d.
FLRT3-LRR F160A 1000 n.d. n.d. n.d.