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. Author manuscript; available in PMC: 2017 Mar 29.
Published in final edited form as: Cell Rep. 2016 Mar 17;14(12):2901–2911. doi: 10.1016/j.celrep.2016.02.066

Fig. 3. Codependence of thioredoxin-1 and the SPI2 type III secretion system.

Fig. 3

Survival of wild-type (WT) and mutant Salmonella in primary macrophages from C57BL/6 (B6) or gp91phox-deficient mice 12 h after challenge (A). p < 0.001 when compared to spiC or trxA mutant controls. Competitive index of ΔspiC ΔtrxA::km, ΔtrxA and ΔspiC Salmonella (B). The competitive index in livers and spleens was estimated 7 d after C57BL/6 mice (n= 7–10) were challenged i.p. with ~105 CFU of an equal mixture of the indicated strains. Intracellular growth of ΔtrxA (C) and ΔspiC (D) Salmonella in J774 cells. The effect of trxA- or trxC-expressing pTRXA or pTRXC plasmids on the intracellular growth of ΔtrxA Salmonella was also evaluated (C). The data are the mean ± SD of 3 independent experiments. *, p < 0.05. Confocal microscopy of GFP-expressing ΔtrxA Salmonella and CI-MPR+ lysosomes in HeLa cells (E). In the merged panel, Salmonella, lysosomes, and cell host nucleus are seen green, red, and blue, respectively. White scale bar = 1 µm. See also Fig. S2, and movies S1–S3.