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. Author manuscript; available in PMC: 2016 Jul 1.
Published in final edited form as: Dev Biol. 2016 May 3;414(2):149–160. doi: 10.1016/j.ydbio.2016.04.028

Fig. 3.

Fig. 3

Comparison of Bhlhb5::flpo and Bhlhb5::cre alleles. (A) Strategy to compare the recombination from Bhlhb5::flpo and Bhlhb5::cre alleles using the RCE family of reporters. The RCE::dual allele contains both FRT-flanked and LoxP-flanked stop sequences. Upon cre-mediated removal of a stop sequence, a Flp-dependent reporter is generated (RCE::FRT) that can be used to visualize recombination from the Bhlhb5::flpo allele. Alternatively, upon Flp-mediated removal of a stop cassette in the RCE::dual allele, a cre-dependent reporter is generated (RCE::LoxP) that can be used to visualize recombination from the Bhlhb5::cre allele. (B) Coronal sections of Bhlhb5::flpo; RCE::FRT mice at P12 immunostained for GFP reveals restricted recombination a subset of cells in the DCN. Scale bar = 100 µm (C) Coronal sections of adult Bhlhb5::cre; RCE::LoxP mice at P12 immunostained for GFP reveals widespread recombination throughout the DCN. Scale bar = 100 µm (D–E) Sagittal sections from mice at P12 showing GFP expression in DCN and cerebellum from Bhlhb5::flpo; RCE::FRT (E) and Bhlhb5::cre; RCE::LoxP (F) mice. Scale bar = 200 µm. (F–G) Transverse sections showing GFP expression in the lumbar spinal cord from adult Bhlhb5::flpo; RCE::FRT (F) and Bhlhb5::cre; RCE::LoxP (G) mice. Scale bar = 100 µm.