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. Author manuscript; available in PMC: 2016 Jul 1.
Published in final edited form as: Dev Biol. 2016 May 3;414(2):149–160. doi: 10.1016/j.ydbio.2016.04.028

Fig. 6.

Fig. 6

Pax2-, Tbr2- and CamKIIα-expressing neurons are reduced in the DCN of Bhlhb5 mutant mice. (A–B) Coronal sections from mice at P8 showing the DCN in control (Bhlhb5flpo/+; RCE::FRT) or Bhlhb5 mutant (Bhlhb5flpo/−; RCE::FRT) mice stained with antibodies to Pax2. (C) Quantification of (A–B) showing a significant reduction in the number of Pax2-expressing cells in the dorsal cochlear nucleus in Bhlhb5 mutant mice relative to controls. (D–F) Immunostaining (D, E) and quantification (F) of Tbr2-positive neurons in the DCN of P16 mice showing a significant loss of neurons in Bhlhb5 mutant mice relative to controls. (G–I) Immunostaining (G–H) and quantification (I) of CamKIIα-expressing neurons, again showing a significant decrease in the absence of Bhlhb5. Representative confocal optical sections are shown. Scale bar = 200 µm. For quantification, n = 3–4 DCN from control/mutant littermate pairs. Data are presented as mean + SEM and * indicates a significant difference relative to controls (P < 0.05, t-test). All counts were conducted blind to genotype.