Fig. 2.
Inhibition of SOCE attenuates the detrimental effects of H2O2 on EPCs. Representative immunoblots (top) and quantification (bottom) of STIM1 expression 48 h after transfection (A). EPCs that had been cultured for 7 days were pretreated with ML-9 for 20 min or with STIM1 shRNA before the addition of H2O2 for 6 h. Cell viability (B) and apoptosis (C) were then measured. The relative expression levels of caspase 12 (D) and caspase 9 (E) were measured by Western blot analysis. *p<0.05; #p<0.05.