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. 2016 Jul 1;24(4):380–386. doi: 10.4062/biomolther.2015.154

Fig. 2.

Fig. 2.

GSK3β-mediated proteasomal degradation of β-catenin by silymarin treatment. (A, B) HCT116 and SW480 cells were pretreated with 10 μM of MG132 or 20 μM of SB216763 for 2 h and then co-treated with 100 μM of silymarin for 6 h. Cell lysates were subjected to SDS-PAGE and then the Western blot was performed for β-catenin and actin. (C) HCT116 cells were treated with 100 μM of silymarin for the indicated times. Cell lysates were subjected to SDS-PAGE and then the Western blot was performed for phospho-GSK3β and total-GSK3β. *p<0.05 compared to cells without silymarin.