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Effect of aloe-emodin on MAP kinase activation in ATDC5 cells. Cells were cultured in a serum free media for 16 h, and then treated with 10 μM aloe-emodin or 10 μg/ml insulin for indicated times. The cells extracts were using specific antibodies against the phosphorylated forms of the MAPKs and analyzed by Western blot. These data were compared to data obtained with antibodies directed against the unphosphorylated states of the kinase.
