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Effect of MAP kinase inhibitor on chondrogenesis with aloe-emodin in ATDC5 cells. ATDC5 cells were treated with aloe-emodin and seeded at a density of (5×104) cells per well for 14 days in the presence or absence of 20 μM PD 98059 (A). Stained cells were dissolved in 10% acetic acid for subsequent quantification of the absorbance at 650 nm (B). Each histogram represents the mean ± S.E.M. (n=3).
