Expression of pri-miR17-92 is increased in culture-activated rat HSCs and in rodent models of alcohol-induced injury. (A, B) pri-miRNA17-92 expression in culture-activated Day 10 (D10) vs day quiescent (DQ) HSCs (A) (*p < 0.05 vs DQ) and in rats fed Lieber-DeCarli alcohol diet (EtOH) and Lieber-DeCarli alcohol diet with biweekly intra-peritoneal injections of lipopolysaccaride (LPS, 0.5mg/kg, ELPS) compared to pair-matched Control diet (B) (*p<0.05 vs Control; #p<0.05 vs EtOH). (C, D) Kinetics (0, T0; 2, T2; 6, T6; 12, T12; 18, T18; 24, T24; 48, T48; 72, T72 hours after media change) of miR19b (C) and pri-miR17-92 (D) expression in primary HSCs exposed to HepG2E47 conditioned media (CM) with 0 (white bars) or 25 (black bars) mM alcohol. Cells collected at time of media change were considered T0 (*p < 0.05 vs T0 with 25mM CM; #p < 0.05 vs T0 with 0mM CM; **p<0.05 compared between T18 and T24)