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. Author manuscript; available in PMC: 2017 Jul 1.
Published in final edited form as: Alcohol Clin Exp Res. 2016 Jun 13;40(7):1430–1442. doi: 10.1111/acer.13116

Fig. 7.

Fig. 7

AAV2-mediated expression of miR19b inhibited expression of MeCP2 and altered expression of miR processing p68 in HSCs exposed to alcohol conditioned media. Primary rat HSCs transduced with AAV2 control or AAV2 miR19b (5000 VG/cell for 24 hours) were exposed to conditioned media from HepG2E47 cells containing 0 (E47CM0) or 25 (E47CM25) mM alcohol for 24 hours. (A, B) MeCP2 mRNA (A) and protein (B) expression (*p < 0.05 vs AAV2 Control E47CM0; **p < 0.05 vs AAV2 Control E47CM25). (C) p72 (left panel) and p68 (right panel) mRNA expression (*p < 0.05 vs AAV2 Control E47CM0; **p < 0.05 vs AAV2 Control E47CM25). (D) Representative immunoblots for p68 and p72 protein (right panel) and densitometry of Western blots (left panel) (*p < 0.05 vs AAV2 Control E47CM0; #p < 0.05 vs AAV2 Control E47CM25).