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. Author manuscript; available in PMC: 2017 Jul 1.
Published in final edited form as: Alcohol Clin Exp Res. 2016 Jun 13;40(7):1430–1442. doi: 10.1111/acer.13116

Fig. 8.

Fig. 8

AAV2-mediated expression of miR19b altered localization, cellular or exosomal, of individual miRs in the miR17-92 cluster. Primary rat HSCs transduced with AAV2 control or AAV2 miR19b (5000 VG/cell for 24 hours) were exposed to conditioned media from HepG2E47 cells containing 0 (E47CM0) or 25 (E47CM25) mM alcohol for 24 hours. Cells and tissue culture media (TCM) were collected for exosome isolation and RNA was extracted from isolated exosomes and cells for qRT-PCR. (A) Cellular expression of individual miRs in the miR17-92 cluster from cells exposed to E47CM25 (*p < 0.05 vs AAV2 Control). (B) Exosomal expression of individual miRs in the miR17-92 cluster (*p < 0.05 vs AAV2 Control). (Cells exposed to E47CM0 can be found in Supplemental Fig. 3)