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. Author manuscript; available in PMC: 2017 Jun 30.
Published in final edited form as: Cell. 2016 May 26;166(1):209–221. doi: 10.1016/j.cell.2016.05.011

Figure 5. GPR65 neurons respond in vitro to an HTR3A agonist.

Figure 5

(A) Calcium responses by Fura-2 imaging of dissociated vagal sensory neurons from Gpr65GFP/+ mice to CCK-8 (10 nM), the HTR3A agonist m-chlorophenylbiguanide (mCPB, 100 μM) and capsaicin (1 μM), scale bar: 40 μm. Top: Fura-2 excitation; bottom: GFP fluorescence (green) and calcium responses (magenta). (B) Representative responses of a GPR65 neuron. (C) Pie chart indicating percentage of GPR65 neurons activated (red) by each ligand. (D) Two color FISH in vagal ganglia. Scale bar: 100 μm. See also Figure S6.