Fig. 5.

Transplantation of co-SG cells in vivo. a, b HE-stained images. c, d PAS-stained images. Regeneration of tissue (asterisk) in mice that differs in the vicinity of intrinsic glands within the same capsule. Asterisk regeneration of tissue, m submandibular gland, l sublingual gland. e–g Immunostained images. DAPI (blue), e human-specific mitochondria (green), f amylase (red), and g AQP-5 (red, mouse-specific antibody used) merged images of mitochondria and amylase, confirming the tissue was formed from human cells. Asterisk regeneration of tissue, m salivary gland of mouse. h RT-PCR showed expression of salivary gland markers. Primers for human-specific sequences were used