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. 2016 Jun 8;19:373–388. doi: 10.1007/s10456-016-9513-x

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© The Author(s) 2016

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.

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Fig. 2 — Anti-angiogenic effect of first (FTB) and third (TTB) trimester trophoblast-conditioned medium (CM) on the vessel formation of the chicken chorioallantoic membrane (CAM). CAMs were treated with on-plants (silicone rings) containing FTB CM, TTB CM or control medium. TTB CM reduced the tertiary and quaternary vessels (white arrows). Per condition, four different eggs were treated, each performed with six on-plants. a Representative pictures of each condition immediately after application of the silicone ring on day 0 and at day 3 of the treatments are shown. b Data were scored on day 3 of the treatment