Fig. 3.

Expression and secretion of PEDF from first (FTB) and third (TTB) trimester trophoblasts. a Quantification of PEDF mRNA in FTB and TTB by real-time qPCR using the expression of the ribosomal protein RPL30 as internal control. b Quantification of PEDF in FTB versus TTB CM of measured with ELISA. c PEDF-neutralizing antibody significantly reduced the inhibitory effect of TTB CM on network formation of feto-placental endothelial cells. Data are given as mean ± SEM. Statistical analysis used the mean of the triplicate of the number of individual biological replicates. n (real-time qPCR) = 5, n (ELISA) = 8, n (network formation) = 3. Control medium (C) = DMEM/EBM + 7.5 % FCS for 48 h at 37 °C without cells