Figure S7.

Cis-Regulatory Elements for Axonal Translation, Related to Figure 7

(A) Lists of sequence motifs enriched in 5′UTRs, 3′UTRs and alternative exons of axon-enriched mRNAs / exons.

(B) An example of axon-enriched motifs. The scatterplot compares the normalized mRNA levels (log2(FPKM)) between the axonal (y axis) and the retinal (x axis) translatome at stage P0.5 for genes with (red dots) and without (black dots) the motif. The density plot shows the distribution of log2 (axon (FPKM) / retina (FPKM)).

(C) GO enrichment analysis for entire genome containing axon-specific sequence motifs (K: G or T; R: A or G; Y: C or T; M: A or C; R: A or G; and H A or C or T) and their relative efficiency in axonal mRNA translation measured by fluorescence recovery after photobleaching (FRAP) of motif-containing reporter constructs (myr-d2EGFP). Several axon-specific motifs were able to promote mRNA translation in the growth cone relative to a control myr-d2EGFP construct without a UTR. Statistical significance of FRAP compared to the no-UTR control was tested across all time-points (0-10mins) using a two-way ANOVA (from the top bar, n = 16, 5, 5, 7, 8, 7, 3, 8, 8, 8, 5, 8, and 6, respectively). For representative purposes, the mean fluorescence recovery at 10 min post-photobleaching is shown. Error bars represent SEM. ^∗∗p < 0.01, and ^∗∗∗p < 0.001 compared to no-UTR control.