Table 3.

Immunological correlates of AQP4-IgG binding patterns

	Loop A-dependent pattern A (n = 37)	Loop A-independent pattern B (n = 10)	Area under the curve (95 % CI)	p value*
AQP-M23 binding	4.52 (3.57–5.48)	6.02 (3.86–8.19)	0.50 (0.25–0.75)	0.999
AQP4-M1 binding	1.42 (1.01–1.83)	1.78 (0.59–2.97)	0.56 (0.33–0.78)	0.999
AQP4-M1 (% M23)	25.1 (19.3–31.1)	28.1 (10.0–31.5)	0.78 (0.59–0.97)	0.405
AQP4-mycA binding	1.95 (1.69–2.21)	5.66 (4.12–7.19)	0.99 (0.96–1.00)	<0.001
AQP4-mycA (% M23)	24.5 (18.5–30.52)	88.6 (80.4–96.8)	1.00 (1.00)	<0.001
AQP4-delA3 binding	2.15 (1.75–2.56)	5.42 (3.77–7.07)	0.96 (0.90–1.00)	<0.001
AQP4-delA3 (% M23)	28.7 (21.9–35.5)	86.2 (77.0–95.4)	0.97 (0.92–1.00)	<0.001
AQP4-mycC binding	1.36 (1.32–1.41)	1.49 (1.38–1.60)	0.88 (0.72–1.00)	0.045
AQP4-mycC (% M23)	11.1 (7.4–14.8)	20.6 (8.7–32.5)	0.72 (0.52–0. 93)	0.999
AQP4-N153Q binding	3.47 (2.86–4.07)	5.42 (3.63–7.20)	0.75 (0.58–0.92)	0.750
AQP4-N153Q (% M23)	61.3 (49.1–73.5)	89.6 (74.2–100)	0.72 (0.55–0.89)	0.999
AQP4-mycE binding	1.14 (1.11–1.17)	1.29 (1.05–1.53)	0.63 (0.33–0.92)	0.999
AQP4-mycE (% M23)	5.8 (3.7–7.9)	3.7 (2.0–5.5)	0.54 (0.36–0.71)	0.999
AQP4-H230G binding	2.35 (1.80–2.89)	3.94 (2.22–5.67)	0.71 (0.52–0.91)	0.999
AQP4-H230G (% M23)	31.3 (25.0–38.2)	53.6 (35.7–71.4)	0.74 (0.59–0.90)	0.870

AQP4-M23, AQP4-M1, and extracellular loop A, C, and E mutations were expressed in HEK-293A cells and assayed to categorize pattern A and pattern B serum samples as indicated in Fig. 3. Data are shown as means with 95 % confidence interval. Binding binding ratios

*The area under the curve was analyzed by receiver operator curve (ROC) analysis and p values were corrected for 15 comparisons