Figure 3.
RacGAP1-knockdown results in cytokinesis failure. A, representative fluorescent images showing actin (green) and Hoechst (blue) staining of SUM149 cells with or without knockdown of ArhGAP11A or RacGAP1. Scale bar = 20 μm. Arrowheads in merged images indicate binucleated cells. B-C, quantification of the percentage of bi/multinucleated (B) SUM149 or (C) HCC1937 cells with or without knockdown of ArhGAP11A or RacGAP1 (n = three independent experiments in each case ±SEM, ≥ 108 total cells/condition/experiment). Statistical significance, relative to the NS control, was determined by one-way ANOVA with Dunnett's post-hoc test.