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. Author manuscript; available in PMC: 2017 Jul 1.
Published in final edited form as: Cancer Res. 2016 May 23;76(13):3826–3837. doi: 10.1158/0008-5472.CAN-15-2923

Figure 5.

Figure 5

ArhGAP11A and RacGAP1 regulate cell spreading and migration. A, shown in the upper panel are representative fluorescent images of actin (green) and Hoechst (blue) staining of SUM149 cells, with or without knockdown of ArhGAP11A or RacGAP1, after 2 h on 10 μg/ml fibronectin-coated coverslips. Scale bar = 20 μm. Shown in the lower panel is quantification of mean cell area (n = three independent experiments ±SEM, ≥85 cells/condition/experiment) after spreading on fibronectin, normalized to NS control. Statistical significance was determined by one-sample t test relative to the NS control. Multinucleated cells were excluded from the analysis. B, mean random migration velocity of SUM149 cells, with or without knockdown of ArhGAP11A or RacGAP1, on fibronectin-coated coverslips, as measured from 24 h time-lapse movies. Data shown were pooled from three independent experiments, ≥110 total cell tracks/condition ±SEM. Statistical significance, relative to the NS control, was determined by one-way ANOVA with Dunnett's post-hoc test.