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. Author manuscript; available in PMC: 2017 Jul 1.
Published in final edited form as: Cancer Res. 2016 Apr 27;76(13):3802–3812. doi: 10.1158/0008-5472.CAN-15-2498

Figure 2. SIRT2 physically interacts with PKM2.

Figure 2

(a) Flag-SIRT2 or pGFP plasmid were transiently transfected into HEK-293T cells. Total protein extracts were IPed with either anti-PKM2 or Flag antibodies and subsequently separated by SDS-PAGE followed by western blotting with an anti-PKM2, Flag, or tubulin antibody. (b) Endogenous PKM2 or SIRT2 were IPed from HeLa cell lysates and separated via SDS-PAGE followed by western blotting with anti-PKM2 and SIRT2 antibodies. (c) Endogenous SIRT2 and PKM2 were stained by anti-SIRT2 and -PKM2 antibodies in HeLa cells (upper panel). HA-SIRT2 and Flag-PKM2 were co-transfected into HeLa cells (lower panel), stained with anti-HA and FLAG antibodies, and subsequently visualized by confocal microscopy. Scale bar: 30 μm. Representative images are shown. All experiments were done in triplicate.