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. Author manuscript; available in PMC: 2017 Jul 1.
Published in final edited form as: Cancer Res. 2016 Apr 27;76(13):3802–3812. doi: 10.1158/0008-5472.CAN-15-2498

Figure 5. The PKM2 deacetylation mimetic mutants inhibit tumor growth.

Figure 5

(a–c) Sirt2−/−-MMT cells were infected with shPKM2 and re-infected with Flag-SIRT2-WT, Flag-PKM2-WT, Flag-PKM2K305R, or Flag-PKM2K62R/K305R. These cell lines were subsequently used for (a, top panel and b) representative images of cell proliferation assays (doubling time) and (a, middle, low panel and c) growth in soft agar assays, as measured by colony formation (invasion assay). Scale bar = 100 μm. *p < 0.05, **p < 0.01. (d) Sirt2−/−-MMT cells were infected with shPKM2 and re-infected with Flag-SIRT2-WT, Flag-PKM2-WT, Flag-PKM2K305Q, or Flag-PKM2K62R/K305Q. These cells were used for cell proliferation assays (top panel) and soft agar assays (low panel). Experiments were repeated three times and data expressed as mean ± SEM. Representative images are shown.