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. Author manuscript; available in PMC: 2017 Sep 1.
Published in final edited form as: Neurobiol Dis. 2016 May 1;93:226–242. doi: 10.1016/j.nbd.2016.04.010

Figure 6. Primary astrocytes express PAR1 in vitro.

Figure 6

Photomicrographs show PAR1 expression by (A) ALDH1L1-, or (B) GFAP-immunoreactive astrocytes in cell culture. (C) Histogram shows the percent of total DAPI+ cells in each case. 98.5% of all of the DAPI+ cells were positive for the pan astrocyte marker ALDH1L1, indicating that astrocyte cultures are of high purity. 74% of all of the DAPI+ cells were positive for the marker of astrocyte reactivity, GFAP. PAR1 immunoreactivity was localized to 96% of the DAPI+ cells. Examples of doubly labeled cells are provided at arrow and singly labeled cells at arrow head. (*P < 0.05, NK). Scale bars A, B = 50 µm.