Table 1. Primers used for quantitative real-time PCR.
Real time PCR assays used to quantify molecular changes in gene transcription in response to experimental contusion-compression SCI. Unless otherwise indicated, all primers were obtained from Integrated DNA Technologies. (AB, Applied Biosystems).
| Gene | Accession Number |
Primer Sequence Forward/Reverse |
|---|---|---|
| BIM | NM_207680 | Probe Assay ID: Mm.PT.56a.8950841.g |
| GFAP | NM_010277.2 | GCAGATGAAGCCACCCTGG/ GAGGTCTGGCTTGGCCAC |
| IL-6 | NM_031168.1 | Probe Assay ID: Mm00446190_m1 (AB) |
| IL-10 | NM_010548.2 | Probe Assay ID: Mm00439614_m1 (AB) |
| IL-1β | NM_008361.3 | Probe Assay ID: Mm.PT.51.17212823 |
| Neurocan | NM_007789.3 | Probe Assay ID: Mm.PT.56a.10993411 |
| Neurosin (Klk6) |
NM_011177.2 | CCTACCCTGGCAAGATCAC/ GGATCCATCTGATATGAGTGC |
| PAR1 | NM_010169.3 | CTTGCTGATCGTCGCCC/ TTCACCGTAGCATCTGTCCT |
| Rn18s | NR_003278.3 | Probe Assay ID: Mm03928990_g1 (AB) |
| TGF-β1 | NM_011577.1 | Probe Assay ID: Mm01178820_m1 (AB) |
| Thrombin | NM_010168.2 | CTGAACCTGCCCATTGTA/ TTCACAAGCATCTCCTCG |
| TNF | NM_013693.2 | Probe Assay ID: Mm00443258_m1 (AB) |
| VIM | NM_011701.4 | Probe Assay ID: Mm.PT.53a.8720419 |