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. Author manuscript; available in PMC: 2017 Sep 1.
Published in final edited form as: Neurobiol Dis. 2016 Apr 9;93:1–11. doi: 10.1016/j.nbd.2016.03.022

Figure 10. Knockdown of Numb reverses the lysosomal targeting of APP in Par3-depleted cells.

Figure 10

(a) APPwt N2a cells were transfected with indicated constructs then live labeled with APP 6E10 antibody. Cells were fixed at 60 min after internalization and immunostained for internalized APP (red) and the lysosomal marker LAMP1 (green). Scale bar: 10µm.

(b) Quantification of colocalization between internalized APP and LAMP1. Data were expressed as Mean ± SEM with Student's t test, *** p<0.001. n=5–10. Double KD: double knockdown of Numb and Par3.