(a) APPwt N2a cells were transfected with the indicated Par3 and Numb constructs. Cell lysates were immunoprecipitated with anti-APP 6E10 monoclonal antibody and immunoprecipitated complexes were analyzed by Western blot.
(b) Quantification of blots in (a), normalized to GFP-Numb input, *p<0.05, **p<0.01, n=3.