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. Author manuscript; available in PMC: 2017 Sep 1.
Published in final edited form as: Neurobiol Dis. 2016 Apr 9;93:1–11. doi: 10.1016/j.nbd.2016.03.022

Figure 2. Par3 regulate APP processing and Aβ generation.

Figure 2

(a) N2a cells stably expressing WT APP (APPwt) were transfected with plasmids encoding myc tag vector control (Myc vector), myc-Par3b, luciferase shRNA or Par3 shRNA, respectively. 72 h after transfection, cell were lysed, total protein was extracted and subjected to Western blot analysis with the indicated antibodies. APP was probed with A8717 antibody. mAPP: mature APP; imAPP, immature APP. (b) Cortical neurons were infected with lentivirus expressing Venus vector control (Venus), Venus-Par3b, Luciferase shRNA or Par3 shRNA. Five days after infection, media supernatants were collected for detecting sAPPα using the 6E10 antibody. Neurons were lysed, total protein was extracted and subjected to Western blot analysis with the indicated antibodies. (c) Quantification of C-terminal fragment α of APP (CTFα) normalized to mature APP, n=3. (d) Quantification of soluble APPα (sAPPα) normalized to mature APP, n=3. (e, f) Cortical neurons were infected with lentivirus expressing the indicated constructs. ELISA assay was used to measure (e) secreted Aβ40, or (f) intracellular Aβ40 normalized to total protein levels, n=9. Data were expressed as Mean ± SEM with Student's t test: *p<0.05; **p<0.01.