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. Author manuscript; available in PMC: 2017 Sep 1.
Published in final edited form as: Neurobiol Dis. 2016 Apr 9;93:1–11. doi: 10.1016/j.nbd.2016.03.022

Figure 5. Colocalization of internalized APP with the early endosomal marker Rab5.

Figure 5

APPwt N2a cells were transfected with indicated constructs together with GFP-Rab5 (green) and then live labeled with APP 6E10 antibody. Cells were fixed at 20 (a) or 60 min (b) after internalization and immunostained for internalized APP (red) and TGN46 (blue). (c) Quantification of colocalization between internalized APP and Rab5. Data were expressed as Mean ± SEM with Student's t test, *p<0.05. n=10–15. Scale bar: 10µm.