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. Author manuscript; available in PMC: 2017 Sep 1.
Published in final edited form as: Neurobiol Dis. 2016 Apr 9;93:1–11. doi: 10.1016/j.nbd.2016.03.022

Figure 8. Knockdown of Numb rescues the decrease in CTFα in Par3-depleted cells.

Figure 8

(a) Knockdown of Numb in N2a cells. N2a cells were transfected with pSUPER constructs encoding either an shRNA targeting luciferase (control) or Numb. 72 hours after transfection, cells were lysed and subjected to Western blot using the indicated antibodies.

(b) N2a cells stably expressing WT APP (APPwt) were transfected with plasmids encoding shRNA targeting luciferase (Control), Numb shRNA, Par3 shRNA, or both Numb and Par3 shRNAs [Double knockdown (KD)], respectively. 72 h after transfection, cell were lysed, total protein was extracted and subjected to Western blot analysis.

(c) Quantification of blots in b. Data were expressed as Mean ± SEM with Student's t test: *p<0.05; ** p<0.01, ***p<0.001, n=3.