Table 2.

Summary of ITC analysis of the interactions between PP2A B56γ1 and phosphorylated or phosphor-mimicking BubR1 proteins/peptides

Well protein	Titration	N (Sites)	ΔH (kcal/mol)	TΔS (kcal/mol)	K d (μmol/L)
B56γ-WT	BubR1-WT	1.08 ± 0.03	−11.57 ± 0.42	−4.25	2.91 ± 0.50
	BubR1-3D	1.02 ± 0.02	−28.36 ± 0.83	−20.87	2.27 ± 0.37
	BubR1-SDD	1.03 ± 0.02	−10.40 ± 0.22	−2.98	2.40 ± 0.25
	BubR1-DSD	1.19 ± 0.01	−21.50 ± 0.35	−14.25	3.37 ± 0.29
	BubR1-DDT	1.12 ± 0.01	−19.42 ± 0.27	−12.11	3.09 ± 0.22
	BubR1-SDT	1.14 ± 0.02	−11.28 ± 0.23	−4.28	5.24 ± 0.37
	BubR1-DST	1.14 ± 0.02	−9.16 ± 0.27	−1.71	2.33 ± 0.37
	BubR1-SSD	1.16 ± 0.03	−14.78 ± 0.53	−7.72	4.61 ± 0.52
	KKLSPIIEDSREATH	1.02 ± 0.02	−12.06 ± 0.34	−4.71	2.80 ± 0.30
	KKLpSPIIEDSREATH	1.02 ± 0.01	−9.55 ± 0.20	−0.82	0.25 ± 0.06
	KKLSPIIEDpSREATH	0.99 ± 0.02	−11.08 ± 0.31	−2.92	0.69 ± 0.11
	KKLSPIIEDpSREApTH	1.02 ± 0.01	−10.73 ± 0.18	−2.41	0.52 ± 0.06
B56γ-R188A	KKLpSPIIEDSREATH	1.07 ± 0.03	−6.75 ± 0.27	−0.84	1.86 ± 0.30
B56γ-R201A	KKLSPIIEDpSREATH	N/B	N/B	N/B	N/B

B56γ1-WT indicates wild-type human B56γ1 (residues 30–380). All BubR1 proteins listed contain human BubR1 residues 647–720, except for the 15-mer BubR1 peptides (corresponding to BubR1 residues 667–681), in which pS and pT represent phosphor-serine and phosphor-threonine, respectively

N/B: no detectable binding