Fig. 1.

Acteoside decreased the level of ROS in porcine oocytes matured in vitro with acteoside supplementation. The relative ROS production was detected in oocytes treated with a 0 μM (control), b 10 μM, c 30 μM, and d 50 μM acteoside by fluorescence microscopy after 44 h IVM (60 oocytes per group). e The relative intensity of fluorescence was measured using NIS-Elements BRsoftware 3.00. Different superscripts above each bar represent significant difference (P < 0.05). The data shown represent the mean ± standard error of results from four independent experiments