Fig. 3. Expression of caspase-3 cleaved tau and knock-down of sorcin increase thapsigartin-induced cytotoxicity but over-expression of sorcin decreases thapsigartin-induced cytotoxicity.

HEK293 cells were transiently transfected with T4 (A, B) or T4C3 (C, D) constructs in the absence or presence of sorcin overexpression or sorcin siRNA. After 48 hrs of transfection, cells were treated 2.5 µM thapsigartin. Cell viability was measured with MTT assay (A, C) and statistical analyses of cell viability were determined at 48 hr after thapsigartin treatment (B, D). Data were obtained from independent experiments (n=3) and expressed as mean±SD. ^*p<0.05 and ^**p<0.01 indicate significant differences between the indicated groups. Vec stands for vector (pcDNA3.1-), Sor for sorcin, Cont for control, and Tg for thapsigargin.