Fig. 4. Overexpression of sorcin significantly attenuates thapsigartin-induced apoptosis in T4C3 cells.

Representative immunoblots (A) of examined proteins and quantitative analyses of cleaved PARP (B) and cleaved caspase-12 (C). HEK293 cells were transiently transfected T4 or T4C3 constructs and then co-transfected with sorcin or vector, respectively. After 48 hrs of transfection, cells were treated 2.5 µM thapsigartin for 24 hr. Western blotting was carried out with cleaved PARP and cleaved caspase-12 antibodies to determine role of sorcin on thapsigartin-induced apoptosis. Data were obtained from independent experiments (n=3) and expressed as mean±SD. ^*p<0.05 and ^**p<0.01 indicate significant differences between the indicated groups. Vec stands for vector (pcDNA3.1-), Sor for sorcin, Cont for control, and Tg for thapsigargin.